Fig 1: CIIlow does not regulate mitochondial bioenergetics. a Oxygen consumption rate (OCR) in MDA231 sublines was followed during sequential additions of oligomycin, FCCP, and combination with rotenone and antimycin. b–e Basal respiration, maximal respiration, spare respiratory capacity, and ATP production. f CI- and CII-dependent respiration was evaluated in permeabilized cells using the Oxygraph. g Mitochondria isolated from MDA231 sublines were subjected to NBGE followed by WB analysis using antibodies against NDUFA9, NDUFV1, NDUFS8 (CI), Core I (CIII), and COXVa (CIV) and ATP5B (CV). h MDA231 sublines were analyzed for subunits of OXOPHOS complexes using WB after SDS-PAGE. i MDA231 sublines grown in media containing either glucose or galactose were evaluated for proliferation using the xCELLingence instrument. Data shown are mean values ± SD (n = 3); images and graphs in i are representative of three independent experiments